Skip to main content
. 2008 Apr;20(4):827–842. doi: 10.1105/tpc.107.056457

Figure 2.

Figure 2.

MS Analysis of Purified H3 and H4 Histones.

(A) Total ion chromatograms for the tryptic digestion mixture of histone H4 from UV-B–treated (top panel) or no UV-B (bottom panel) samples. Insets show FT-MS spectra of an acetylated N-terminal tail peptide.

(B) Structure and tandem mass spectrum of the acetylated peptide, 4-GKacGGKacGLGKacGGAKacR-17.

(C) Average ratios of peak areas integrated from LC-MS runs of representative peptides detected from the duplicates of UV-B–treated and no UV-B histone H4. The peptide 4-GKacGGKacGLGKacGGAKacR-17 is more abundant in the UV-B–exposed sample. Values are from two independent experiments, and error bars indicate se.

(D) Average ratios of peak areas integrated from LC-MS runs of representative peptides detected from the duplicates of UV-B–treated and no UV-B histone H3. The peptide 9-KacSTGGKacAPR-17 is more abundant in the UV-B–exposed sample. Values are from two independent experiments, and error bars indicate se.