Figure 3.

Acetylation State of Histones H3 and H4 Associated with the mbd101 and nfc102 Loci.

ChIP assays utilized antibodies specific for N-terminal acetylated histone H3 ([A]; AcH3), N-terminal acetylated H4 ([B]; AcH4), or total histone H4 ([C]; H4) in nuclei prepared from W23 or Confite with UV-B treatment (UV-B) and control conditions (no UV-B). The immunoprecipitates were analyzed for the presence of promoter and transcribed sequences of the target genes mbd101 and nfc102 and a transcribed sequence of a control gene that is not UV-B–regulated (th-like) by quantitative PCR. ChIP data were normalized to input DNA before immunoprecipitation. No signal was detected in control reactions (performed for each sample reaction reported above) incubated in the absence of any antibody. Quantitative RT-PCR analysis assessed transcript levels (mRNA) in control and UV-B–treated plants (D). Fifty nanograms of cDNA after reverse transcription of RNA was used. All measurements were done at least in triplicate for each independent preparation. Error bars indicate se. Statistically significant differences between the UV-B and control treatments (Student's t test; P = 0.05) are labeled with asterisks.