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. 2008 Jun 6;4(6):e1000081. doi: 10.1371/journal.ppat.1000081

Figure 4. Purified TDM from ΔmmaA4 mutant stimulated macrophages to produce IL-12p40.

Figure 4

(A) Bone marrow-derived macrophages were treated with 5 µg of purified M. tuberculosis TDM (wild type TDM) or mutant TDM (ΔmmaA4 TDM). Cytokines in conditioned media were harvested at 22 and 44 hr post-TDM treatment. Supernatants were analyzed for the presence of IL-12p40 and TNF-α by ELISA. Vehicle treatment is the solvent in which the TDM was dissolved. Statistically significant differences for ΔmmaA4 TDM compared to wild type TDM are indicated by asterisks as follows: *, p<0.05 (*); **, p<0.01; ***, p<0.001 (two-way ANOVA, Bonferroni post-tests). Values are the means±SD of triplicate samples and are representative of 3 separate experiments performed on 2 independent batches of purified TDMs. (B) Dose responses to TDM purified from wild type M. tuberculosis H37Rv (wild type TDM) or ΔmmaA4 mutant (mmaA4 TDM). Bone marrow-derived macrophages were treated with varying doses of TDM purified from wild type or ΔmmaA4 mutant M. tuberculosis. Conditioned media were assayed for cytokines at 44 hr by ELISA. Symbols as in (A); values are the means±SD of triplicate samples and are representative of 2 separate experiments performed on 2 independent batches of purified TDMs.