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. Author manuscript; available in PMC: 2008 Jul 15.
Published in final edited form as: Cancer Res. 2007 Jul 15;67(14):6637–6646. doi: 10.1158/0008-5472.CAN-07-0751

Figure 2. uPA silencing suppressed invasion and angiogenesis in PC3 prostate cancer cells.

Figure 2

(A) Comparison of the in vitro invasive potentials of cells transfected with mock, siMM or siuPA in the presence or absence of 5-aza and TSA. Data are mean ± SD three independent experiments (p<0.01).(B) Representative invasion photographs from cells transfected with mock, siMM or siuPA as described in (A).(C) The PC3-derived conditioned medium significantly increased EC tube-like network formation in culture, an effect nearly completely blocked by neutralizing antibodies to either uPA or VEGF.(D) Angiogenic activity in media conditioned by uPA knockdown or control cell culture using the EC tube-like formation assay. Data are mean ± SD three independent experiments (p<0.01). Representative angiogenic activity photographs in media conditioned by uPA knockdown or control cell cultures are shown below.