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. Author manuscript; available in PMC: 2008 May 21.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Aug 25;226(1):94–106. doi: 10.1016/j.taap.2007.08.015

Figure 2.

Figure 2

Total and phosphoprotein staining of immunocaptured complex I. Representative, paired-gel lanes of immunocaptured complex I from HepG2 cells run by PAGE and stained first with Pro-Q® Diamond phosphoprotein gel stain (P lanes) followed by SYPRO® Ruby protein stain (S lanes) as described in materials and methods. HepG2 isolated mitochondria were incubated in phosphorylation buffer (see methods) with the indicated NRTI for 30 minutes before complex I was immunocaptured as described. Pro-Q® stained phosphoproteins of apparent molecular weight of 95, 64, 23.9, and 14.5 kDa were resolved. The 14.5 kDa band (indicated by arrow) showed cAMP/PKAc dependent phosphorylation.