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. 2008 Jun;14(6):1228–1236. doi: 10.1261/rna.964708

FIGURE 1.

FIGURE 1.

Formation of a specific complex between A3G and HIV-1 NC. (A) 293T cells were transfected with plasmids expressing A3G-Myc-His, GFP-Myc-His, Luc-Myc-His, or A3A-Myc-His. At 44 h after transfection, the cells were lysed and the lysates incubated with recombinant purified GST (lane 1) or GST-NC (lanes 2–5). Bound complexes were then collected using glutathione agarose beads. Input lysates (upper panel, 5% of total) and bound proteins (middle panel, 25% of total) were then visualized by Western analysis using an anti-Myc polyclonal antiserum. GST fusion proteins and non-fused GST were visualized by Western analysis using an anti-GST polyclonal (lower panel). (B) Similar to panel A, except that the 293T cells were transfected with plasmids expressing HA-tagged forms of A3A, A3G, A3G-E1, or A3G-E1+2. GST-NC bound proteins were collected using glutathione agarose beads and input lysates (5% of total) and bound proteins (25% of total) visualized by Western analysis using an HA-specific mouse monoclonal antibody. GST-NC (lower panel) was visualized as described in panel A.