FIGURE 5.

Mutations in noncanonical A598-A662 base pair cause partial suppression of dG interference at position 840 in D5. (A) Autoradiographs of sequencing gels after iodine cleavage of precursor (pre) and branched (br) RNAs. Selection step (branching reaction) was carried out in the presence of wild-type 5′-end labeled D56 RNA transcribed in the presence of dG phosphorothioate and unlabeled wild-type and mutant exD123 RNAs. Numbers on the left indicate guanosine positions in D5. (B) A diagram comparing relative intensity of bands corresponding to G840 in precursor and branched products. The results represent average values obtained from at least three independent experiments; the error did not exceed 15%.