FIGURE 2.

Functions of RBP16 domains in RNA annealing. (A) RNA annealing reactions were performed as in Fig. 1 with radiolabeled A6U5 pre-mRNA and gA6[14]NX gRNA, using the indicated amounts of wild-type (WT) and F14,16A RBP16. (B) Representative RNA annealing reactions performed in the presence of the indicated amounts of full-length (FL) RBP16, RBP16 CSD, and RBP16 RGG domain proteins. Replication protein A (RPA) was used as a negative control. (*) An alternative A6U5 pre-mRNA secondary structure that is sometimes observed. The graphs represent the mean and standard error of three separate experiments.