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RBP16 stimulation of gRNA/pre-mRNA annealing does not require the gRNA oligo(U) tail. Representative RNA annealing assays were performed as in Fig. 1 with radiolabeled A6U5 pre-mRNA and either A6 gRNA with a 17-nt oligo(U) tail (gA6[14]NX) or the same gRNA without an oligo(U) tail (gA6[14]NT). MRP2 was used as a positive control. (WT) Wild type. The graphs represent the mean and standard error of three separate experiments.
