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Features of the RNA limit annealing stimulation. Representative annealing assays were performed as in Fig. 1 except using a truncated A6 pre-mRNA (A6U5 41 nt). The sequence for A6U5 41 nt is highlighted in gray in Fig. 1A. MRP2 was used as a positive control and p22 protein was used as a negative control. (WT) Wild type. The graphs represent the mean and standard error of three separate experiments.
