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Identification of the DcpS nuclear import sequences. Hela cells transfected with either (A) wild-type Flag-DcpS or the (B) mutant construct containing a four amino acid truncation of amino acids 10–13 Flag-DcpSΔKR. The left panel demarcates the position of the nuclei with DAPI staining and the DcpS localization is denoted by the green fluorescence and identified with the use of the anti-Flag monoclonal antibody. A merge of the two panels is shown on the right.
