Skip to main content
. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Neurochem Int. 2007 Oct 18;52(6):962–971. doi: 10.1016/j.neuint.2007.10.010

Figure 3. Analysis of colocalization of NOS-2 and GFAP immunoreactivity.

Figure 3

Astrocyte cultures were treated with both TNFα and TGFβ1 as described in Figure 2 and exposed to LPS plus IFNγ for 14 hr, after which NOS-2 and GFAP immunoreactivity was assessed by immunofluorescence microscopy. Representative fluorescent images for NOS-2 (green), GFAP (red) and DAPI (blue) were obtained from the same microscopic field and superimposed using Adobe Photoshop. (a) NOS-2 and DAPI; (b) GFAP and DAPI; (c) NOS-2,GFAP, and DAPI. Scale bar = 20 µm.