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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Neurochem Int. 2007 Oct 18;52(6):962–971. doi: 10.1016/j.neuint.2007.10.010

Figure 5. Effect of TGF²1 on TNF± mRNA expression in astrocytes.

Figure 5

Astrocyte cultures were treated with either 3 ng/mL TGFβ1 alone (A) or 2 µg/mL LPS plus 3 ng/mL IFNγ (B) and total RNA was harvested at the times indicated. TNFα and β-actin mRNA levels were assessed via RT-PCR (see “Experimental Procedures”). CON = PCR reaction mix minus cDNA. Results are representative of two separate experiments on cultures from independent dissections.