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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: Cancer Res. 2008 May 15;68(10):3992–3998. doi: 10.1158/0008-5472.CAN-07-6594

Figure 2.

Figure 2

UVB induces CB1 or CB2 phosphorylation and internalization. HEK293 cells transfected with (A) pcDNA4-HisG-CB1 or (B) pcDNA4-HisG-CB2 were irradiated with UVB (4 kJ/m2) followed by incubation at 37°C in a 5% CO2 incubator for 5, 15, or 30 min. The transfected cells not exposed to UVB were used as a negative control. Cells were harvested at the indicated time and the membrane protein was extracted and subjected to immunoprecipitation with a HisG monoclonal antibody. CB1 or CB2 serine phosphorylation was detected by Western blotting with a phospho-serine antibody. The total protein levels of (C) CB1 or (D) CB2 using the same sample as in (A) and (B), respectively, were determined by Western blotting with the HisG monoclonal antibody to show the localization of the CB1 or CB2 receptor in the membrane fraction after UVB treatment.