Table 1.
Precocious phenotype of activated lin-12 gain-of-function (gf) mutant worms and interactions with the let-7 heterochronic pathway components.
| % of animals with adult lateral alae | ||||
|---|---|---|---|---|
| Genotype | L4 larva (L3 molt) | Adult (L4 molt) | Alae phenotype | Other phenotypes |
| N2 (wild-type) | 0% (48)* | 100 % (56) | Normal | - |
| lin-12(n137gf) | 35% (60) | 100% (30) | Precocious | Muva, Eglb |
| lin-12(n137n460gf) | 54% (106) | 100% (37) | Precocious | Mltc, Muv, Egl Dpyd |
| lin-12(n427gf) | 51 % (80) | 100% (29) | Precocious | Muv, Egl |
| Lin-12(427gf), lag-1(om13lf) | 0% (17) | 100% (20) | Normal | Egl, Embe, Lvaf |
| lin-12(n676gf) | 30% (40) | 100 % (33) | Precocious | Egl |
| lin-12(n302gf) | 29% (31) | 100% (10) | Precocious | Egl |
| lin-12(n676n930lf) | 0% (30) | 100% (10) | Normal | Egl, Vulg |
|
| ||||
| let-7(n2853lf) | 0% (20) | 0% (20) | Retarded | Mlt, Egl |
| let-7(mn112null); unc-3(e151lf) | 0% (20) | 0% (20) | Retarded | Mlt, Let |
| lin-41(ma104lf) | 46% (57) | 100% (25) | Precocious | Mlt, Egl, Dpy |
| lin-29(n333lf) | 0% (15) | 0% (31) | Retarded | Mlt, Egl, |
|
| ||||
| let-7(n2853lf); lin-12(n137n460gf) | 0% (20) | 0% (20) | Retarded | Mlt, Muv, Egl |
| let-7(mn112null); lin-12(n137n460gf) | 0% (16) | 0% (25) | Retarded | Mlt, Muv, Egl |
| lin-41(ma104lf); lin-12(n137n460gf) | 50% (32) | 100% (16) | Precocious | Mlt, Muv, Egl |
| lin-29(n333lf); lin-12(n137n460gf) | 0% (30) | 0% (25) | Retarded | Mlt, Muv, Egl |
*
Number animals scored.
a
Multi-vulva,
b
Egg-laying defect,
c
Molting defect,
d
Dumpy,
e
Embryonic lethal,
f
Larval arrest,
g
Vulvaless.