Figure 3. Loss of Gata1, Gata2 and Biklf has differing effects of krcp, kiaa0650 and glcci1 expression.

(A) In control embryos at 12 somites, glcci1, kiaa0650, and krcpare expressed in ICM blood cell precursors. Expression of glcci1 in the ICM precursors was not lost in biklf (100%, n=21), gata1/biklf (100%, n=43), gata2/biklf (100%, n=7) or gata2/biklf MO-injected gata-/- embryos (100%, n=5). Similarly, kiaa0650 expression was not absent in biklf (100%, n=18), gata1/biklf (100%, n=63), or gata2/biklf (100%, n=9) injected embryos, but was greatly reduced in gata2/biklf MO-injected gata-/- embryos (70%, n=10). krcp was expressed in biklf (100%, n=10) and gata2/biklf (70%, n=20), but unlike glcci1 not in biklf MO-injected gata1-/- embryos (100%, n=11) or gata2/biklf MO-injected gata-/- embryos (100%, n=2). (B) While reduced in biklf/gata2 MO-injected and biklf/gata2 MO-injected gata-/- embryos, draculin expression was present in all MO-injected embryos (100%, n=54). scl, lmo2 and gata2 are expressed in both the anterior and posterior blood and endothelial cell precursors in wild type embryos. Expression of scl (100%, n=25), lmo2 (100%, n=27) and gata2 (100%, n=28) was maintained in biklf MO-injected and biklf MO-injected gata-/- embryos. scl (100%, n=33), lmo2 (100%, n=40) and gata2 (97%, n=31) expression was reduced in biklf/gata2 MO-injected and biklf/gata2 MO-injected gata-/- embryos. Expression of biklf was reduced in the blood precursors and absent in the hatching gland of biklf MO-injected, biklf/gata2 MO-injected, biklf MO-injected gata-/-, and biklf/gata2 MO-injected gata-/- embryos (100%, n=63). znfl2 expression was maintained in biklf MO-injected embryos (100%, n=18), reduced in biklf/gata2 MO-injected (100%, n=26) and biklf MO-injected gata-/- embryos (100%, n=4), and virtually absent in biklf/gata2 MO-injected gata-/- embryos (100%, n=10).