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. Author manuscript; available in PMC: 2009 Mar 28.
Published in final edited form as: J Mol Biol. 2008 Jan 31;377(3):845–853. doi: 10.1016/j.jmb.2008.01.064

Figure 3. M. thermautotrophicus GatCAB can transamidate Glu-tRNAAsn.

Figure 3

Phosphorimage of a TLC separating Glu-[32P]AMP, and Gln-[32P]AMP released from M. thermautotrophicus tRNAAsn by digestion with nuclease P1 following incubation with either no enzyme (lane 1), GatDE (200 nM) (lane 2) or GatCAB (200 nM) (lane 3), for 7 minutes at 37 °C in the presence of ATP (4 mM), 32P labeled M. thermautotrophicus Glu-tRNAAsn (1 µM), and amide donor (4 mM). The M. thermautotrophicus GluRS was only able to glutamylate up to ten percent of the M. thermautotrophicus tRNAAsn. The amide donor in the GatCAB reaction was Gln and the donor in the GatDE reaction was Asn.