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. Author manuscript; available in PMC: 2009 Apr 18.
Published in final edited form as: Cell. 2008 Apr 18;133(2):340–353. doi: 10.1016/j.cell.2008.01.052

Figure 1.

Figure 1

Rac1 activation is required for canonical Wnt signaling. (A-C) Western analyses to detect GTP-bound forms and total amounts of Rac1, Cdc42 and RhoA, in ST2 cells cultured in Wnt3a versus L conditioned medium (C. M.) for 0.5 or 1 hr. The relative amount of the GTP-bound form normalized to the total amount, in L medium, is designated 1.0. (D) Rac1 activation by purified recombinant Wnt3a protein (rWnt3a) at 50 ng/ml. (E) Expression of Lef1-Luciferase in cells infected with a control virus (IE, expressing GFP) or viruses expressing dnRac1 or dnCdc42. The cells were first infected with the viruses, then transiently transfected and cultured in regular growth medium for 47 hrs, and finally incubated in Wnt3a or L conditioned medium for 1 hr before being harvested. (F) Western analyses of Rac1 in cells at ∼96 hrs after transfection with control (Ctrl) or Rac1 siRNA. (G) Expression of Lef1-Luciferase following siRNA transfections. (H-I) Expression of AP following viral infection (H) or siRNA transfection (I). (J-K) Western analyses of β-catenin in cytosolic (J) or nuclear (K) fractions of cells cultured in L or Wnt3a medium for 1 hr following viral infections. Cytosolic and nuclear signals were normalized to GAPDH and CREB-1, respectively. *: p<0.05, n=3.