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. Author manuscript; available in PMC: 2009 Apr 18.
Published in final edited form as: Cell. 2008 Apr 18;133(2):340–353. doi: 10.1016/j.cell.2008.01.052

Figure 5.

Figure 5

Rac1 and JNK activity are required for β-catenin nuclear localization in response to Wnt. ST2 cells were infected with indicated viruses and cultured in L or Wnt3a medium for 1 hr, with or without SP600125 (10μM), before being subjected to immunofluorescence confocal microscopy. (A-F) Nuclei as revealed by NLS-EGFP expressed by each virus via IRES. (A’-F’) Endogenous β-catenin detected by immunostaining. Yellow arrow in D’: enrichment of β-catenin at a cell-cell junction. White arrow in E’ and F’: enrichment of β-catenin in certain areas of the cell periphery. Note characteristic “fried-egg” cellular morphology in D’ and F’, caused by expression of caRac1. (A“-F”) Merged pictures of GFP and β-catenin signals.