Figure 3.

Infection status regulates chemokine receptor expression. Whole blood was collected and screened for CXCR1 (panel A) or CCR2 (panel B) by staining with PE-conjugated antibodies specific for each receptor and then analyzing by flow cytometry gating on neutrophils (panel A) or CD14⁺ monocytes (panel B). Patients were divided into those without infection over the preceding 12 mo (inf−) and those having more than three infections over the preceding 12 mo (inf⁺). Subgroup analyses were performed using the Kruskal–Wallis nonparametric test followed by pairwise multiple comparisons using the Mann–Whitney test as described in the Statistical Analyses section. Significances are indicated as (*) P < 0.0042 versus control, (**) P < 0.0035 versus control.