Figure 11.

Gross morphology and Vegf expression in podocytes of E13.5 metanephric explants treated with BMP4 or Noggin. Metanephroi harvested from E13.5 wild-type mice were treated with vehicle (A and D), BMP4 (B and E), or Noggin (C and F) for 48 h. (B) The metanephros treated with BMP4 developed poor expansion of condensed mesenchyme, leading to lack of mesenchyme around some tips of ureteric buds (arrowheads). (C) The explant treated with Noggin was morphologically indistinguishable from the control explant (A). (D through F) Bright field images of Vegf in situ hybridization are shown. The expression level of Vegf was comparable among the control (D), BMP4-treated (E), and Noggin- treated explants (F). Bar = 300 μm in A through C. Magnification, ×200 in D through F.