Table 3.

Primer sequences and expected product sizes^a

Gene	Accession No.	5′ to 3′ Sequence	Size (bp)
Rat P2X1	BC061742	S: GAAGTGTGATCTGGACTGGCACGTAS: GCGTCAAGTCCGGATCTCGACTAA	452
Rat P2X2	NM_053656	S: CGACGACTGTATTGCCGAS: CTCCAATGACACCGCC	374
Rat P2X3	NM_031075	S: TGGCGTTCTGGGTATTAAGATCGGAS: CAGTGGCCTGGTCACTGGCGA	440
Rat P2X4	NM_031594	S: GAGGCATCATGGGTATCCAGATCAAGAS: GAGCGGGGTGGAAATGTAACTTTAG	447
Rat P2X5	NM_080780	S: TGTCATTCCATCTCAGGGGGAS: TTCGGCATCCTTTAGAAGGG	286
Rat P2X6	X97376	S: AAAGACTGGTCAGTGTGTGGCGTTCAS: TGCCTGCCCAGTGACAAGAATGTCAA	520
Rat P2X7	NM_019256	S: GTGCCATTCTGACCAGGGTTGTATAAAAS: GCCACCTCTGTAAAGTTCTCTCCGATT	354
Rat P2Y1	NM_012800	S: ACGTCAGATGAGTACCTGCGAS: CCCTGTCGTTGAAATCACAC	289
Rat P2Y2	NM_017255	S: ACTTTGTCACCACCAGCGTGAGAS: TGACGTGGAAAGGCAGGAAG	279
Rat P2Y4	NM_031680	S: TGTTCCACCTGGCATTGTCAGAS: AAAGATTGGGCACGAGGCAG	294
Rat P2Y6	NM_057124	S: TGCTTGGGTGGTATGTGGAGTCAS: TGGAAAGGCAGGAAGCTGATAAC	339
Rat HPRT	NM_012583	S: CTGACCTGCTGGATTACATTAAS: TTTCGCTGATGACACAA	410

^aPrimer sequences taken from Turner et al.,⁶⁰ Katugampola and Burnstock,⁶¹ and Shibuya et al.⁶² S, sense; AS, antisense.