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. 2008 Apr;19(4):743–748. doi: 10.1681/ASN.2007091030

Figure 4.

Figure 4.

(A and B) Time-course analysis of renin-induced (10 nM; A) and prorenin-induces (2 nM; B) ERK1/2 phosphorylation (p-ERK1/2) in human VSMC (top) with unphosphorylated ERK as loading control (bottom). (C) Renin and prorenin (10 min) also induced p-ERK1/2 in AT1A receptor–deficient VSMC. (D through F) Effects of 10 μM aliskiren (D), 1 μM HRP (E), and MEK1/2 inhibitor (PD98059; 100 nM; F) on renin- and prorenin-induced (both 10 min) p-ERK1/2 in human VSMC (top) with unphosphorylated ERK as loading control (bottom). All experiments were performed after 24-h serum starvation in the presence of losartan (Los) and the AT2 receptor blocker (PD123319).