Figure 2.

Caspase-3 immunoblot. Full-length caspase-3 protein expression (32 kDa) was increased in cpk mouse kidneys compared with +/+. Caspase-3 expression was decreased in cpk;casp3^+/− mouse kidneys. The cpk;casp3^+/− mice that had the longest survival (105 d and 113 d) had less expression of caspase-3 than the cpk;casp3^+/− mice that had the shortest survival (29 d and 17 d). Caspase-3 expression was absent in cpk;casp3^−/− mouse kidneys. Representative immunoblot of at least 3 separate experiments (100 μg protein loading per well). Equal protein loading was confirmed by Coomassie Blue staining of membranes.