Figure 5.

Polysomal distribution of specific cellular mRNAs. Polysomal distributions, visualized after sucrose gradient sedimentation, of cellular mRNAs from mock- (Mock) and poliovirus-infected cells at 3 hours after infection (3 Hr PI) are shown. Absorbance profiles at 254 nm of the collected sucrose gradients are shown at the top of each panel. Ten fractions were collected from each gradient (fraction 1 is the top of the gradient), and equal volumes of each fraction were separated on a formaldehyde-agarose gel and subjected to Northern blot analysis using specific radiolabeled DNA probes. The sedimentation profiles of c-Myc, actin, and polioviral (polio) mRNAs have been shown previously (4) and are included as controls in this study.