Figure 2.

Morphologies of breast cell lines cultured in two‐ and three‐dimensions. Phase contrast images of the complete panel of 25 breast cell lines cultured on tissue culture plastic (top panel) and in the 3D lrECM assay (middle panel) are shown grouped by 3D morphological classification: Round, Mass, Grape‐like and Stellate. 3D cultures were stained for F‐actin and nuclei were counterstained with DAPI. Optical sections of representative colonies are shown for all cell lines (bottom panel) with the exception of MDA‐MB‐468 for which a Z projection with maximum intensity projection (Image J) is shown (because of the high degree of dimensionality of the colonies generated by this cell line, optical sections appear to show single cells not evidently part of the same colony). Scale bars: top panel, 100μm; middle panel, 50μm; bottom panel, 20μm.