Table 1.
Cross-reactive CTL From BALB/c Mice Inoculated With LCMV or Vectors Expressing LAS NP‡
| Percent Specific Lysis of Infected Clone 7 Cells† |
|||||
|---|---|---|---|---|---|
| Immunization* | E:T | Uninfected | VV-WT | VV-LASNP | LCMV |
| Salm-LasNP | 12.5 | 0.6 ± 0.4 | 0.5 ± 0.9 | 4.0 ± 1.6 | 7.6 ± 0.9 |
| 25 | 2.1 ± 0.4 | 3.3 ± 0.3 | 9.6 ± 0.8 | 13.8 ± 1.0 | |
| 50 | 2.3 ± 0.4 | 3.3 ± 0.2 | 14.8 ± 0.4 | 17.1 ± 0.7 | |
| Ctrl. Salm. | 12.5 | 1.9 ± 1.0 | 1.4 ± 1.1 | 1.3 ± 1.2 | 2.4 ± 0.4 |
| 25 | 1.0 ± 1.1 | 1.5 ± 1.4 | 1.2 ± 0.7 | 2.6 ± 0.6 | |
| 50 | 1.3 ± 1.0 | 1.9 ± 0.2 | 0.5 ± 0.9 | 4.0 ± 0.9 | |
| VV-LASNP | 12.5 | 2.2 ± 1.0 | 1.3 ± 1.1 | 6.4 ± 1.2 | 7.5 ± 0.4 |
| 25 | 1.9 ± 1.1 | 3.6 ± 1.4 | 9.3 ± 0.7 | 11.3 ± 0.6 | |
| 50 | 2.8 ± 1.0 | 3.8 ± 0.2 | 14.5 ± 0.9 | 16.5 ± 0.9 | |
| LCMV | 12.5 | 2.3 ± 0.3 | 4.3 ± 0.3 | 10.2 ± 1.0 | 35.9 ± 3.2 |
| 25 | 1.9 ± 0.1 | 3.9 ± 0.6 | 14.0 ± 0.7 | 46.0 ± 4.9 | |
| 50 | 3.4 ± 0.6 | 4.9 ± 0.5 | 16.5 ± 1.7 | 69.3 ± 1.8 | |
*
BALB/c mice, two to three per group, were immunized by intragastric intubation and sacrificed 4 days after their second inoculation to assess splenocyte CTL activity.
†
Percent specific lysis of uninfected or infected Clone 7 target cells is presented as the average of determinations from two to three individual mice.
‡
These data are very similar but not identical to data presented in our previous publication1; they were done with new groups of mice to obtain parallel proliferation and challenge data.
E:T indicates effector:target cell ratio.