Table 2.
Proliferative Responses of Splenocytes From Immunized and Surviving Mice
| SI to Immunogen† |
||
|---|---|---|
| Immunization* | LCMV (0.5 μg/mL) | LAS NP (0.5 μg/mL) |
| VV-LASNP | ||
| Mouse 1 | 6.0 | 7.2 |
| Mouse 2 | 29.3 | 16.2 |
| Mouse 3 | 24.9 | 8.4 |
| Salm-LasNP | ||
| Mouse 1 | 23.0 | 18.2 |
| Mouse 2 | 21.4 | 8.5 |
*
Splenocytes were taken from mice immunized and surviving as described in the Methods section and Table 1 and were assessed for ability to proliferate to LAS or LCMV antigens.
†
SI were calculated as described in the Methods section, and indicate uptake of 3H-thymidine after 6 days of antigen stimulation. In this case, antigens were recombinant LASNP (0.5 μg/mL) or heat-inactivated LCMV (0.5 μg/mL). Each line represents data from a single mouse. Splenocytes from mice inoculated with Ctrl.Salm. or mock-infected had no proliferative responses (ie, SI values of <5.0).