Figure 2.

(A) Fractional sodium excretion in 80-d-old (7/5) and 120-d-old (7/7) 11βHSD2^−/− mice (▪) and age-matched C57BL/6J mice (□) (B) The absolute effect of amiloride on sodium excretion (Δ amiloride). (C) The renal expression of ENaC-α mRNA normalized to the expression of 18S RNA. (D) Expression of ENaC-α protein in the CCD of 120-d-old 11βHSD2^−/− mice, showing that immunoreactivity is predominately cytoplasmic. Western analysis shows the relative expression of SGK1 (E) and Nedd4 (F) in the kidney (n = 6 for each group) of 11βHSD2^−/− mice, as a ratio of that in kidneys of age-matched C57BL/6J mice. The dotted line shows the level of equal expression. Protein quantification was by densitometry. All data are means ± SEM. Comparisons were made using ANOVA with Bonferroni post hoc test or, for E and F, a one-sample t test assuming a sample mean of 1.0. *P < 0.05; **P < 0.01.