Table 1.

Plasma and urine osmolality (mOsm/kg H₂O); free water clearance (μ l/min); and plasma concentrations of potassium (mmol/L), aldosterone (nmol/L), and corticosterone (nmol/l) in four cohorts of 11βHSD2^−/− and C57BL/6J mice^a

Parameter	Age (d)
	35	60	80	120
POsm
C57BL/6J	305 ± 3	304 ± 3	312 ± 2	306 ± 7
11βHSD2−/−	315 ± 3b	311 ± 2b	313 ± 4	295 ± 2
UOsm
C57BL/6J	1801 ± 116	1630 ± 240	2007 ± 170	1572 ± 75
11βHSD2−/−	779 ± 67c	647 ± 45c	604 ± 99c	609 ± 40c
FWC
C57BL/6J	−5.9 ± 0.9	−5.8 ± 0.7	−3.6 ± 0.6	−4.1 ± 0.5
11βHSD2−/−	−3.1 ± 0.7b	−3.2 ± 0.6b	−2.2 ± 0.6b	−1.6 ± 0.3c
PAldo
C57BL/6J	1.57 ± 0.24	1.60 ± 0.02	1.31 ± 0.18	1.07 ± 0.09
11βHSD2−/−	0.63 ± 0.12c	0.44 ± 0.07c	0.69 ± 0.07c	0.45 ± 0.05c
PCort
C57BL/6J	374 ± 90	323 ± 20	338 ± 80	NA
11βHSD2−/−	286 ± 58	289 ± 35	242 ± 22	NA

^aData are means ± SE. Statistical comparisons were made using ANOVA with least significant difference post hoc test. ANOVA indicated significant effect of genotype in all four variables. There was no effect of age per se for any variable, but there was a significant interaction between age and genotype for U_Osm (P < 0.01), FWC (P < 0.01), and P_K (P < 0.01). FWC, free water clearance; P_Aldo, plasma concentration of aldosterone; P_Cort, plasma concentration of corticosterone; P_K, plasma concentration of potassium; P_Osm, plasma osmolality; U_Osm, urine osmolality

^bP < 0.05.

^cP < 0.01.