Figure 1.

Nestin staining in glomerular crescents at different stages. Podocytes along the glomerular tuft are positive for nestin; the glomerular and parietal basement membranes are highlighted by PAS staining. (A) The earliest change noted was bridging of visceral podocytes to the parietal basement membrane (arrow). (B) Bridging could also be seen to nestin-positive cells along the parietal basement membrane (arrow). (C) Mixtures of nestin-positive and nestin-negative cells were found along the parietal basement membrane (arrows). (D) Crescents extending from the glomerular tuft were a mixture of nestin-positive and nestin-negative cells (arrow). (E) Nestin-positive cells comprised a variable proportion of crescentic cells, with up to 50% positive in some crescents. Arrow points to the residual glomerular basement membrane within this large crescent. (F) Nestin-positive cells were still seen in organized fibrous crescents. The collapsed glomerular tuft with nestin-positive podocytes is marked by an arrow. Immunoperoxidase with PAS counterstain, original magnifications: B, C, D, E, ×200; A, F, ×400.