Figure 2.

HIF-1α and HIF-1α target gene mRNA levels are reduced by cisplatin. (A) RNase protection assays show that HIF-1α mRNA was reduced dose-dependently in HKC-8 cells after cisplatin treatment compared with controls or vehicle (DMF)-treated cells. Expression of the HIF target gene Glut-1 was also reduced by cisplatin. DP treatment demonstrated up-regulation of Glut-1 mRNA. (B) Up-regulation of Glut-1 is reduced dose-dependently after HKC-8 cells were pretreated with cisplatin, demonstrating the impaired responsiveness of the HIF system in the presence of cisplatin. Representative data of 3 independent experiments. U6sn mRNA was used as loading control.