Figure 6.
Systemic immunity against sheep Ig is not affected by DC depletion. (A) Serum samples from DT-injected CD11c-DTR/GFP mice (black bars) and controls (white bars) from the experimental protocol shown in Figure 4D were taken on day 14 and analyzed by ELISA for titers of total murine IgG, IgG1, and IgG2a levels specific for sheep IgG (mouse IgG/IgG1/IgG2a α sheep IgG). (B) 2 × 105 splenocytes from the same mice were cocultured with 10 μg/ml sheep Ig. After 3 d, concentrations of IL-10 and TNFα and, in separate experiments, of IL-2 and IFNγ, in the culture supernatant were determined by ELISA. n.s, not significant.