Figure 2.

Histology of kidney tissues from mice after sham operation, 30- or 60-min renal ischemia and transcriptional contribution of leukocytes to kidney tissue transcriptomics. (A) 30-min sham, (B) 60-min sham, (C) 30-min ischemia and 6-h reperfusion, (D) 60-min ischemia and 6-h reperfusion, (E) 30-min ischemia and 36 h reperfusion, and (F) 60-min ischemia and 36-h reperfusion. Comparing sham kidney with normal histology, both 30- and 60-min ischemic kidneys displayed cast formation and tubular injury at 6- or 36-h postischemia. Sixty-minute ischemic kidney had more injury than 30-min ischemia at each time point. Hemotoxylin and eosin stain. Arrows indicate injured tubules. Open arrows indicate debris and cast formation. (G and H) Transcriptional changes of leukocyte-specific Cd11 gene. Compared with the sham operated controls, the overall contribution of leukocytes to kidney transcriptomics is moderate (5 to 30%). IRI, ischemia reperfursion injury.