Figure 3.

Histology of lung tissues from mice after sham operation, 30- or 60-min renal ischemia, and transcriptional contribution of leukocytes to lung tissue transcriptomics. (A) 30-min sham, (B) 60-min sham, (C) 30-min ischemia and 6-h reperfusion, (D) 60-min ischemia and 6-h reperfusion, (E) 30-min ischemia and 36-h reperfusion, and (F) 60-min ischemia and 36-h reperfusion. Compared with the sham-operated controls, 30-min renal ischemia induced mild lung changes with septal edema and hypercellularity at 6 h, which resolved by 36-h postischemia. Meanwhile, 60-min renal ischemia induced lung changes at 6 h that persisted throughout the 36-h reperfusion period. (G and H) Moderate (10 to 50%) transcriptional changes of Cd11 gene with the highest leukocyte contribution to lung tissue transcriptomics at 36 h after 60 min of kidney ischemia.