Figure 4.

12/15 lipoxygenase (12/15-LO) short hairpin RNA (shRNA) can reduce AT1R expression in mesangial cells (MC). (A) RMC were transfected with empty vectors and vectors expressing 12/15-LO or scrambled shRNA sequence. 12/15-LO and AT1R protein expression were determined by Western blotting. (B) Bar graph quantification showing significant reduction in relative AT1R expression in 12/15-LO shRNA-transfected RMC compared with scrambled shRNA or empty vector. Data represent mean ± SEM of four independent experiments (^#P < 0.01 versus scrambled control).