Figure 7.

Role of p38MAPK in 12(S)-HETE-induced AT1R. (A) Quiescent RMC were pretreated with p38MAPK inhibitor SB202190 (10⁻⁶ M for 30 min), then stimulated with 0.1 μM 12(S)-HETE for 6 h, and AT1R protein expression was determined by Western blotting. (B) Bar graph representation of data in A. Results shown are representative of three experiments (^#P < 0.01 versus control; ^##P < 0.01 versus 12(S)-HETE). (C) RMC were preincubated with SB202190, then with 12(S)-HETE for 6 h, followed by actinomycin D and then total mRNA extracted at the indicated time periods. AT1R mRNA levels remaining at each time point were quantified by real-time PCR (mean ± SEM, n = 3).