Figure 8.

12(S)-HETE pretreatment can amplify Ang II signaling responses in RMC. (A) Quiescent RMC pretreated with 12(S)-HETE (10⁻⁷ M) for 24 h, and then stimulated with Ang II (10⁻⁷ M) for 5 min. Cell lysates were immunoblotted with specific antibodies to phosphorylated and nonphosphorylated forms of ERK1/2, p38MAPK and STAT3. (B through D) Densities of protein bands were quantified and presented as fold over control. Ang II can induce ERK1/2, p38MAPK, and STAT3 signaling activation, and 12(S)-HETE pretreatment enhances these effects of Ang II (*P < 0.05; ^#P < 0.01 versus control; **P < 0.05 versus Ang II, n = 3).