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Expression of mouse CYP2C55 in liver of Cyp3a+/+ and Cyp3a−/− mice as detected by Western blotting. 30 μg of microsomal protein was loaded per lane. Recombinant CYP2C55 enzyme, migrating at 50 kDa (arrow), was used as positive control (0.5 pmol/lane). Total protein staining (Ponceau S and India Ink) confirmed equal loading across the lanes (not shown).
