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. 2008 Apr 8;98(10):1696–1703. doi: 10.1038/sj.bjc.6604326

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Copyright © 2008 Cancer Research UK

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Reverse transcriptase-PCR to investigate expression of the two gastrin transcripts in a panel of GI cancer cell lines: (1) PAN1, (2) BXPC3 (pancreatic); (3) HCT116, (4) HT29 (5) C170HM2 (colonic); (6) ST16, (7) MGLVA1 (gastric); (8) OE19 and (9) OE21 (oesophageal). cDNA (+) and a negative control in which the RT was omitted (−) were amplified using a primer specific for each transcript ((A) alternative transcript and (B) Ensembl transcript) and a common primer. The expected position of the bands is indicated by an arrow based on the position of the 100-bp markers (M).