Abstract
Inhibition of growth of axenic cultures of Nitrosomonas europaea by nitrapyrin was investigated in liquid culture and in soil. In liquid culture, exponentially growing cells were more sensitive than stationary-phase cells, possibly due to a requirement for uptake of nitrapyrin, metabolism of nitrapyrin, or both before inhibition. Differences in sensitivity were observed between the parent strain and two strains, sp1 and sp2, that were selected through repeated subculturing. These differences were reflected in the length of the lag period induced by nitrapyrin and in the specific growth rate and were due to different bactericidal and bacteriostatic effects. Soil provided significant protection from inhibition, with concentrations of nitrapyrin approximately one order of magnitude greater than those required for equivalent inhibition in liquid culture. The data show that strain differences alone do not explain differences in sensitivity between nitrification in soil and in liquid culture and suggest that the inhibitor may be more effective against actively nitrifying soils.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Belser L. W., Schmidt E. L. Inhibitory effect of nitrapyrin on three genera of ammonia-oxidizing nitrifiers. Appl Environ Microbiol. 1981 Mar;41(3):819–821. doi: 10.1128/aem.41.3.819-821.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CAMPBELL N. E., ALEEM M. I. THE EFFECT OF 2-CHLORO, 6-(TRICHLOROMETHYL) PYRIDINE ON THE CHEMOAUTOTROPHIC METABOLISM OF NITRIFYING BACTERIA. I. AMMONIA AND HYDROXYLAMINE OXIDATION BY NITROSOMONAS. Antonie Van Leeuwenhoek. 1965;31:124–136. doi: 10.1007/BF02045882. [DOI] [PubMed] [Google Scholar]
- COCHRAN W. G. Estimation of bacterial densities by means of the "most probable number". Biometrics. 1950 Jun;6(2):105–116. [PubMed] [Google Scholar]
- Hooper A. B., Terry K. R. Specific inhibitors of ammonia oxidation in Nitrosomonas. J Bacteriol. 1973 Aug;115(2):480–485. doi: 10.1128/jb.115.2.480-485.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lees H., Quastel J. H. Biochemistry of nitrification in soil: 1. Kinetics of, and the effects of poisons on, soil nitrification, as studied by a soil perfusion technique. (with an Addendum by H. Lees). Biochem J. 1946;40(5-6):803–815. doi: 10.1042/bj0400803. [DOI] [PMC free article] [PubMed] [Google Scholar]