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. 2008 Jun;131(6):575–587. doi: 10.1085/jgp.200709924

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© 2008 Jia et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Single-channel analysis of NGF action. (A) Single-channel records at 0 mV from a cell-attached patch containing one KCNQ/M channel, before, during, and after bath application of 3 μM Oxo-M and 20 ng/ml NGF. Five consecutive 1-s sweep are shown for each condition. Bars: 100 ms, 1 pA. (B) The time course of P_o plotted against time for the experiment shown in A. P_o calculated from 5-s periods. The application of Oxo-M and NGF are indicated by the horizontal bars. (C) Single-channel amplitudes derived from all point amplitude histograms were plotted against membrane potentials. The mean slope conductance obtained by fitting a linear regression was 6.5 ± 0.2 and 6.1 ± 0.2 pS (n = 5) in the presence and absence of NGF, respectively. (D) Mean P_o-V_m curves determined in control and in the presence of NGF or Oxo-M. Data points were fitted by a Boltzmann equation, yielding values for P_o,max, V_1/2, and k of (control, ○) 0.44 ± 0.02, −32 ± 3 mV, 12 ± 2 mV; (NGF, ♦) 0.33 ± 0.08, −25 ± 2 mV, 10 ± 2 mV; (Oxo-M, ▾) 0.06 ± 0.005, −29 ± 3 mV, 8 ± 2 mV, respectively. Each data point is the mean ± SEM of 5–7 patches. Error bars show SEM.