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. 2008 May 22;118(6):2347–2364. doi: 10.1172/JCI32914

Figure 2. Effect of FoxO1 on MTP promoter activity.

Figure 2

(A) HepG2 cells in 6-well microplates were cotransfected with pCVM5-LacZ vector plus wild-type or mutant MTP promoter–directed luciferase reporter systems in the presence of Adv-null vector (–) or Adv-FoxO1 vector (+) at a fixed dose (MOI, 200 pfu/cell). After 24-h incubation, the luciferase activities were normalized to β-gal activity and then determined and compared between control and FoxO1 conditions. Data were obtained from 8 experiments. (B) Response of wild-type and mutant MTP promoters to insulin. HepG2 cells were transfected with test plasmids using pCMV-LacZ as a control and transduced with either Adv-null or Adv-FoxO1 vector in the presence and absence of insulin (100 nM). The relative luciferase activity for each construct was determined after 24-h incubation. Data were obtained from 4–9 experiments. *P < 0.001 versus control.