Figure 1.

Adoptively transferred SMARTA cells mimic the endogenous CD4 response to LCMV. a) 1 × 10⁴ CD44^lo SMARTA cells (Thy1.1⁺) were transferred into B6 hosts (Thy1.2⁺). Mice were infected with LCMV one day later, and the subsequent expansion, contraction and memory maintenance of SMARTA cells in the spleen were calculated based on expression of Thy1.1. Error bars display the SEM (n=3-4 per time point) and results are representative of four different experiments. b) Splenocytes harvested at day 8 or day 300 post-infection were restimulated with GP_61-80 peptide for four hours in the presence of Brefeldin A. Cells were then stained for intracellular expression of IFNγ, TNFα and IL-2. We gated on CD4⁺ Thy1.1⁺ SMARTA responders and CD4⁺Thy1.1^- endogenous responders from the same animal and assessed their ability to make each cytokine at each time point. Flow plots are representative of effector and memory time points over the course of four time-course experiments.