Figure 2.

SMARTA cells expand but fail to gain full effector function and form memory after Lm-gp61 infection. a) 1 × 10⁴ CD44^lo SMARTA cells (Thy1.1⁺) were transferred into B6 hosts (Thy1.2⁺). Mice were infected with Lm-gp61 or LCMV one day later, and the expansion, contraction and memory maintenance of SMARTA cells in the spleen were assessed. Representative flow plots display the frequency of Thy1.1⁺CD4⁺ SMARTA cells in the spleen at day 8 and day 60 post-infection for each infection. b) Representative flow plots display the frequency of IFNγ-producing endogenous CD4+ responders (CD4⁺Thy1.1^-) in the spleen following peptide restimulation in the presence of Brefeldin A. c) Representative flow plots compare the ability of SMARTA cells (CD4⁺Thy1.1⁺) and endogenous CD4⁺ responders (CD4⁺Thy1.1^-) in the spleen to make IFNγ, TNFα and IL-2 following peptide restimulation at day 8 post-infection. Flow plots are representative of six separate experiments. d) The number of SMARTA (Thy1.1⁺) and endogenous (IFNγ-producing) responders in the spleen are shown for day 8 and day 60. Error bars are the SEM (n=3/group).