Skip to main content
. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Immunity. 2008 Mar 20;28(4):533–545. doi: 10.1016/j.immuni.2008.02.014

Figure 5.

Figure 5

SMARTA responders demonstrate lower functional avidity, as compared to endogenous CD4 responders to Lm-gp61 in the same host. a) B6 mice were infected with either LCMV or Lm-gp61. Eight days later splenocytes were restimulated over a range of GP61-80 peptide concentrations and stained for expression of IFNγ and TNFα. Representative flow plots are gated on CD4+ T cells and show the frequency of cytokine producers following restimulation at the indicate peptide concentrations. b) The graph displays the number of endogenous CD4+ T cells in the spleen capable of producing IFNγ in response to decreasing concentrations of peptide. c) 1 × 104 CD44lo SMARTA cells (Thy1.1+) were transferred into B6 hosts (Thy1.2+), and mice were infected with LCMV one day later. The graph displays the percent maximal endogenous or SMARTA CD4 response induced by ex vivo peptide restimulation, as measured by the frequency of IFNγ-producing responders at each peptide concentration divided by the frequency of IFNγ-producing responders at the highest peptide concentration (1 × 10-5 M). d) 1 × 104 CD44lo SMARTA cells (Thy1.1+) were transferred into B6 hosts (Thy1.2+), and mice were infected with Lm-gp61 one day later. The graph displays the percent maximal endogenous or SMARTA CD4 response induced by ex vivo peptide restimulation, as measured by the frequency of IFNγ-producing responders at each peptide concentration divided by the frequency of IFNγ-producing responders at the highest peptide concentration (1 × 10-5 M). The error bars are the SEM (n=3 for each group). Results are representative of four separate experiments. e) 1 × 103 CD44lo SMARTA cells (Thy1.1+) were transferred into B6 hosts (Thy1.2+), and mice were infected with LCMV or Lm-gp61 one day later. Decreasing concentrations of gp66-77/I-Ab Class II tetramer was used to stain SMARTA and endogenous responders at day 8 post-infection. The graph displays the number of tetramer-positive endogenous cells detected at each concentration, normalized to staining with the control tetramer hCLIP/I- Ab. f) and g) The graph displays the percentage of tetramer-positive cells as compared to the number of tetramer-positive cells at the highest tetramer concentration following LCMV or Lm-gp61 infection. Error bars are the SEM (n=4/group).