Transformation of cells with oriC chimeric plasmids with only one I-site results in normal colonies. W3110 was transformed with chimeric plamsids containing wt oriC (panel 1), oriCI2/I3ADP (panel 2), oriCI2ADP (panel 3), oriC I3ADP (panel 4), oriC I2/I3ADP/MATP (panel 5), or oriCMATP (panel 6). Colonies are shown after overnight growth.