Figure 1.

Dynamics of nitrotyrosine conversion. Nitrotyrosine (100 nmol) was incubated with 10 mM DTT and 25 μM hemoglobin at pH 7.2 at various temperatures and times as indicated. An equivalent of 180 pmol of nitrotyrosine was analyzed with HPLC-ECD as described in Materials and Methods. The percentage of conversion was calculated by the comparison with the amount of detected nitrotyrosine at time zero in a sample lacking hemoglobin. The detections of amounts <1 pmol were considered 100% conversion. A representative experiment is shown.