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Nitrotyrosine is reduced to aminotyrosine. (A) HPLC-ECD chromatogram showing the resolution of nitrotyrosine (NO2-Y), aminotyrosine (NH2-Y), and tyrosine (Y) standards. (B) A representative chromatogram of the reaction products after nitrotyrosine incubation with hemoglobin and DTT at room temperature or incubation at 100°C with either hemoglobin or DTT alone. (C) The chromatogram of the reaction products after nitrotyrosine incubation with hemoglobin and DTT at 100°C.
