Figure 3.

Nonenzymatic conversion of various nitrophenol compounds. Each nitrophenol compound (1 mM for each) was incubated with 10 mM DTT and 25 μM hemoglobin at pH 7.2 at 100°C for 10 min. The conversion of each compound was analyzed spectrophotometrically at predetermined wavelengths as described in Materials and Methods. Data are presented as percent of conversion (means ± SE). NT, ortho-nitrotyrosine; NA, para-nitroaniline; NC, para-nitrocatechol; o-NP, ortho-nitrophenol; m-NP, meta-nitrophenol; p-NP, para-nitrophenol; BOC-NT, BOC-ortho-nitrotyrosine. The specific wavelengths are indicated in Material and Methods.